Multiple nanoscale phenomena of membranes regulate mesoscale processes in cells, which in turn control macro-scale processes in living organisms.
Using a lipid bilayer for DNA origami assembly, it is possible to perform a hierarchical assembly of multiple DNA origami nanostructures, such as the integration of functional components into a frame architecture.Ĭontinuous, dynamic, and controlled membrane remodeling creates flow of information and materials across membranes to sustain life in all biological systems.
For the control of the nanostructure formation, the photo-controlled assembly and disassembly of DNA origami were performed reversibly, and dynamic assembly and disassembly were observed on a lipid bilayer surface using HS-AFM. Various patterns of lattice–tile complexes were created by changing the interactions between the lattice and tiles. The formed lattices were used to further assemble DNA origami tiles into their cavities. During the diffusion of DNA origami on the membrane surface, the formation of lattices and their rearrangement in real-time were observed using high-speed atomic force microscopy (HS-AFM). Programmed patterns of 1D and 2D DNA origami assembly using specific DNA strands and micrometer-sized lattice assembly using cross-shaped DNA origami were performed on a lipid bilayer surface. The micrometer-scale assembly of various DNA nanostructures is one of the major challenges for further progress in DNA nanotechnology.
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